ISSN 2415-1297 (Online)   ISSN 2415-1300 (Print)
 
             
 
Volume : 26 Issue : 4 Year : 2018
 
Antibiotic Susceptibility and R-Plasmid Mediated Drug Resistance in Staphylococcus Aureus [Med J Islamic World Acad Sci]
Med J Islamic World Acad Sci. 2005; 15(3): 111-116

Antibiotic Susceptibility and R-Plasmid Mediated Drug Resistance in Staphylococcus Aureus

Mostafizur Rahman1, Abdul Hye Khan2, M. Shahjahan1, Dipak K. Paul3, Pervez Hassan4
1Department of Biochemistry, University of Rajshahi, Rajshahi, Bangladesh.
2Department of Genetics and Breeding, University of Rajshahi, Rajshahi, Bangladesh.
3Department of Applied Nutrition and Food Science, Islamic University, Khustia, Bangladesh.
4Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh.

A total of twenty-eight Staphylococcus aureus strains were isolated from skin lesion samples. Results of antibiotic susceptibility test showed that these twenty-eight Staphylococcus aureus strains were resistant to ampicillin (72%), amoxycillin (72%), penicillin (72%), cotrimoxazole (15%), cloxacillin (50%) tetracycline (11%), cephradine (22%), cephalexine (7%) and nalidixic acid (18%). To understand whether this drug resistance phenomenon was plasmid mediated or not, plasmids were isolated from a chosen strain of Staphylococcus aureus (S2) and a 23 KB plasmid was obtained. This 23 KB plasmid was then transferred to an antibiotic sensitive E. coli LE 392 and after which the sensitive E. coli LE 392 strain developed drug resistance. Plasmid analysis of the transformed E. coli LE 392 revealed that it contains a 23 KB plasmid corresponding to that of the donor Staphylococcus aureus strain which may harbor the gene(s) encoding multidrug resistance in the donor Staphylococcus aureus.

Keywords: Plasmid, multidrug resistance, staphylococcus aureus.


Mostafizur Rahman, Abdul Hye Khan, M. Shahjahan, Dipak K. Paul, Pervez Hassan. Antibiotic Susceptibility and R-Plasmid Mediated Drug Resistance in Staphylococcus Aureus. Med J Islamic World Acad Sci. 2005; 15(3): 111-116

Corresponding Author: Abdul Hye Khan, Bangladesh


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