ISSN 2415-1297 (Online)   ISSN 2415-1300 (Print)
 
             
 
Volume : 26 Issue : 4 Year : 2018
 
Extracellular Amylase Synthesis by Aspergillus Flavus and Penicillium Purpurescence [Med J Islamic World Acad Sci]
Med J Islamic World Acad Sci. 1989; 2(4): 272-276

Extracellular Amylase Synthesis by Aspergillus Flavus and Penicillium Purpurescence

Z. A. Olama, S. A. Sabry
From Botany Department, Faculty of Science, Alexandria University, Alexandria, Egypt.

The potentiality of the two mold fungi A. flavus and P. purpurescence to produce extracellular amylase when grown on starch-containing medium as a sole carbon source in shaked flasks was tested. The maximal amylase productivity was achieved after 7 days incubation at 45C. An initial pH value of 7 was found to be the optimum for amylolytic activity. Best activity was achieved upon using 2% (v/v) inoculum with 50 ml medium in 250 ml culture flask The greatest yield of amylolytic activity was obtained when starch or glycogen were used as carbon source. A starch concentration of 15% (w/v) favoured the highest enzyme activity, Ca+2 and Na+ ions showed stimulatory effect on enzyme productivity.

Keywords: Amylase, Aspergillus flavus, Penicillum purpurescence.


Z. A. Olama, S. A. Sabry. Extracellular Amylase Synthesis by Aspergillus Flavus and Penicillium Purpurescence. Med J Islamic World Acad Sci. 1989; 2(4): 272-276

Corresponding Author: Z. A. Olama, Egypt


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