Nuclear Transplantation in Different Families of Teleost: Combination of The Nucleus of Zebrafish (Brachydanio Rerio) and The Cytoplasm of Loach (Paramisgurnus Dabryanus)

In this paper, we present the results obtained in the experiments of nuclear transplantation in teleost of different families. The nuclei of blastula cells of zebrafish (Brachydanio rerio, family Cyprinidae, order Cypriniformes, 2N=50) were transplanted into the enucleated eggs of loach (Paramisgruns dabryanus, family Cobitidae, order Cypriniformes, 2N=48). From 4812 nucleocytoplasmic hybrid (NCH) eggs eight NCH larval fish were obtained (0.17%). One of which developed up to 151 hours. The developmental potential of this kind of NCH eggs was improved as compared to the NCH eggs obtained in other inter-family combinations reported by us previously, i.e. the combination of the nucleus of goldfish (Carassius autratus, family Cyprinidae, order Cypriniformes, 2N=100) and the cytoplasm of loach (Paramisgurnus dabryanus, family Cobitidae, order Cypriniformes, 2N=48) and their reciprocal combination, the combination of the nucleus of loach and the cytoplasm of goldfish. The results obtained in this experiment indicated that the similarity of the chromosome number between the nucleus-donor and cytoplasm-recipient fishes will be helpful for improving the natural developmental incompatibility between the nucleus and the cyptoplasm in an NCH egg, even though they originate from two taxonomically distantly related species of fishes.