ISSN 2415-1297 (Online)   ISSN 2415-1300 (Print)
 
             
 
Volume : 27 Issue : 1 Year : 2019
 
Tocopherol Contents of Pakistani Seed Oils Studied by Normal Phase HPLC [Med J Islamic World Acad Sci]
Med J Islamic World Acad Sci. 1994; 7(1): 34-38

Tocopherol Contents of Pakistani Seed Oils Studied by Normal Phase HPLC

Fazlý Manan
From Department of Agricultural Chemistry and Nutrition, NWFP Agricultural University Peshawar, Pakistan.

A high performance liquid chromatographic (HPLC) method was developed for the quantitative determination of tocopherol vitamers (, T and ) in different seed oils. After saponification the oil samples and extracts in n-hexane, tocopherol vitamers were quantitatively analyzed by HPLC with fluorimetric detector. Assay of tocopherol vitamers added to margarine resulted in •97% recovery, showing high precision of the method. The tocopherol vitamers were eluted isocratically by an eluent containing 95% hexane, 5% diethyl ether, at a pump flow rate of 1.5 ml/min. A silica column (Si-60) with a temperature of 45°C was used. The method developed in this investigation enables better separation of the tocopherol vitamers and their quantitative analysis in seed oils.

Keywords: Tocopherol, Pakistani seed oils, normal phase HPLC, liquid chromatography (HP), vitamin E.


Fazlý Manan. Tocopherol Contents of Pakistani Seed Oils Studied by Normal Phase HPLC. Med J Islamic World Acad Sci. 1994; 7(1): 34-38

Corresponding Author: Fazlý Manan, Pakistan


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