ISSN 2415-1297 (Online)   ISSN 2415-1300 (Print)
 
             
 
Volume : 26 Issue : 4 Year : 2018
 
Comparison of 111In and 99mTC Labelled Monoclonal Antibodies in Normal Mice: Biodistribution Studies and Electrophoretic Analyses of Plasma Samples [Med J Islamic World Acad Sci]
Med J Islamic World Acad Sci. 1988; 1(1): 10-12

Comparison of 111In and 99mTC Labelled Monoclonal Antibodies in Normal Mice: Biodistribution Studies and Electrophoretic Analyses of Plasma Samples

Meral T. Ercan1, Heinz Kriegel2
1Department of Nuclear Medicine, Hacettepe University, Medical School, Ankara, Türkiye.
2Department of Nuclear Biology, Gesellschaft für Straklen and Umwelt forschung, Munich, Federal Republic of Germany

Anti-melanoma monoclonal antibody (Mab) kits were obtained from Sorin, Italy. They were labeled with 111In and 99mTc. The labeling efficiencies determined by thin-layer chromatography (ITLC) were 97% and 99% for 111In-Mab and 99mTc - Mab, respectively. Each radiopharmaceutical was I.V. injected to 6 NMRI mice. Those mice injected with 111 In-Mab were sacrificed at 24 h and 48 h. The other 6 injected with 99m Tc-Mab were sacrificed at 6 h and 24 h. Some blood and organs were taken out for weighing and radioactivity assay. The maximum percent uptake per whole organ was obtained in the liver (5.65-0.09 % at 24 h and 6.14-0.10 % at 48 h). The maximum % uptake per g organ was obtained in kidneys (21.70-0.40 at h and 16.18-1.64 at 48 h). When 99mTc-Mab was used the maximum % uptake per whole organ (11.67-0.32 at 6 h, 5.01-0.07 at 24 h) and per g tissue (73.55-3.36 at 6 h, 27.73-0.94 at 24 h) were obtained in kidneys. Electrophoretic analyses of plasma samples indicated that 111In was transferred from Mab to plasma proteins, which explained the observed higher blood radioactivity levels compared to 99mTc-Mab.

Keywords: Anti-melanoma monoclonal antibody (Mab), 111In, 99mTc, electrophoretic analyses


Meral T. Ercan, Heinz Kriegel. Comparison of 111In and 99mTC Labelled Monoclonal Antibodies in Normal Mice: Biodistribution Studies and Electrophoretic Analyses of Plasma Samples. Med J Islamic World Acad Sci. 1988; 1(1): 10-12

Corresponding Author: Meral T. Ercan, Türkiye


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