Changes in The Biochemistry of The Hemolymph of Plodia Interpunctella after Treatment with Bacillus Thuringiensis

The biochemical changes in the hemolymph of P. interpunctella larvae as a result of treatment with B. thuringiensis showed that the total carbohydrate and protein concentrations were not significantly changed. The electrophoretic patterns of the hemolymph protein showed 10 different bands of which six showed great variation in the control and the treated samples. The change in lipid concentration showed a double fold increase after treatment with B. thuringiensis. The total amino acids, on the other hand, showed no quantitative differences in the hemolymph of normal and treated larvae. Analysis of the hemolymph revealed the presence of 15 amino acids. Serine, proline, glycine, alanine, valine, leucine and lysine exhibited quantitative decrease, while aspartic acid, threonine, isoleucine, tyrosine, phenyl alanine and arginine showed an obvious increase after treatment of the larvae with B. thuringiensis. An increase of sodium, potassium, calcium, magnesium and manganese ions, and a decrease in phosphorous, ferric, zinc and copper ions, were also detected after treatment.