G-6-Pd Çorum: A New Variant of Glucose -6- Phosphate Dehydrogenase

A new variant of glucose-6-phosphate dehydrogenase (G-6-PD) is reported. Severe enzyme deficiency (1% of normal) was found in a 16-year-old Turkish school boy. This enzyme deficiency was associated with chronic haemolytic anaemia. The partially purified enzyme had a slow electrophoretic mobility in both polyacrylamide slab gel and cellulose acetate strip system, normal Km for G-6-P, decreased Km for NADP+, normal heat stability, biphasic pH curve and slightly increased utilization of 2-deoxy glucose-6-P, galactose-6-p, and deamino-NADP+. The utilization of NAD+ by the variant enzyme was increased sixfold. The variant is named G-6-PD Çorum, after the place of origin.