Treatment of Pseudomonas Life Threatening Chronic Suppurative Otitis Media by New Conservative Therapy A Prospective Study 1993-1994

A new phenazinomycin-related antibiotic was isolated as a cultural product of Ps. aeruginosa. This antibiotic possesses antibacterial activity against certain gram-positive and gram-negative bacteria including enteric bacteria and Ps. aeruginosa. This minimum inhibitory concentration (MIC) as determined by the both microdilution assay ranged between 25-65 G/mL.The product yield using a novel fermentation and purification technique was 100-605 G/mL, while by classical methods a yield of only 10-50 G/mL was obtained. The physico-chemical properties of the antibiotic such as melting point, ionization constant and spectrophotometric analysis were determined. Preliminary drug toxicological studies (LD50) were determined in mice but did not show toxic or important undesirable effects even at relatively high concentrations of 400 mG/kg using the intraperitoneal route of administration. Clinically the H1 antibiotic in the form of ear drops (500 G/mL) proved effective in the eradication of 82.6% (71/86) gentamicin-resistant. Ps. aeruginosa chronic middle ear infections with dramatic reduction in morbidity and negligible side effects.