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Volume : 30 Issue : 1 Year : 2023
Effects of Bacterial Endotoxin on Some Metabolites and Enzymes in Rat Serum [Med J Islamic World Acad Sci]
Med J Islamic World Acad Sci. 2005; 15(2): 65-72

Effects of Bacterial Endotoxin on Some Metabolites and Enzymes in Rat Serum

ABD El Rahman A. El Garawany1, Othman A. Al Sagair2, Ezzat S. El Daly3, Kamal A. El Shaikh3, Mahmoud H. El Gebaly4, Ahmed A. Mousa1
1Pyrogen Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt.
2Medical Microbiology Department, Faculty of Science, El Qassim University, EL Qassim, KSA. P.O. Box 237, Bureida 81999.
3Zoology Department, Faculty of Science, Helwan University, Cairo, Egypt.
4National Cancer Institute, Cairo University, Cairo, Egypt.

The effects of bacterial endotoxins (Escherichia coli, Klebsiella pneumoniae, and Salmonella typhimurium) on glucose and blood urea nitrogen (BUN) levels and aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT) and lactate dehydrogenase (LDH) activities were studied. Three groups of rats were injected (1 mg/kg body weight, i.p.) with three types of bacterial endotoxins (E. coli, K. pneumoniae and S. typhimurium) as a single dose. The control group was injected i.p. (1 mg/kg) in 0.9% normal saline. Blood sampling was performed from the orbital vein plexus after 24 and 72 hr of injection. Glucose level was increased significantly after 24 hr of after each 3 solutions of endotoxin. Its level showed non-significant decrease after 72 hr post-treatment. However, endotoxins caused significant increases in BUN, ASAT and LDH at 24 and 72 hr post-treatment. On the other hand, the ALAT activity was significantly decreased after the referred observation periods of endotoxins injection. The variation in serum glucose level after 24 and 72 hr post-treatment may be referred to different reasons. On the other hand, the increase of BUN concentration may be due to the toxic effect of bacterial endotoxins resembling to that occurring in renal damage and impairment of renal function. However, the changes in serum aminotransferases and LDH activities may be due to endotoxins induced hepatic microcirculatory disturbance and to the subsequent liver injury and tissue hypoxia.

Keywords: Endotoxin, enzyme activities.

ABD El Rahman A. El Garawany, Othman A. Al Sagair, Ezzat S. El Daly, Kamal A. El Shaikh, Mahmoud H. El Gebaly, Ahmed A. Mousa. Effects of Bacterial Endotoxin on Some Metabolites and Enzymes in Rat Serum. Med J Islamic World Acad Sci. 2005; 15(2): 65-72

Corresponding Author: Ezzat S. El Daly, Egypt

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