Study of Toxicity and Haemagglutinating Activity of A Purified Intracellular Toxin and Extracellular Crude Toxin Isolated from Pseudomonas AeruginosaShahanara Begum1, M. D. Samsuzzaman2, Iftikhar Ahmed1, Parvez Hassan3, Nurul Absar2, Jalaluddin A. Haq41Department of Microbiology, Rajshahi Medical College, Rajshahi, Bangladesh.
2Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.
3Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh.
4Department of Microbiology, BIRDEM, Dhaka, Bangladesh.
The action of purified intracellular toxin (PIT) from Pseudomonas aeruginosa was examined in brine shrimp lethality bioassay. The LC50 of the PIT was calculated to be 25 g/ml. The PIT agglutinated both albino rat and rabbit erythrocytes more potently than did extracellular crude toxin (ECT). Galactose and Dmannose, however, inhibited the agglutination property of PIT and ECT respectively. Intradermal injection of PIT caused changes on the tissues of rabbit skin at a lower dose than that of ECT. Keywords: Pseudomonas aeruginosa toxin, Brine shrimp nauplli, Hemagglutination, Effect on rabbit tissue.
Shahanara Begum, M. D. Samsuzzaman, Iftikhar Ahmed, Parvez Hassan, Nurul Absar, Jalaluddin A. Haq. Study of Toxicity and Haemagglutinating Activity of A Purified Intracellular Toxin and Extracellular Crude Toxin Isolated from Pseudomonas Aeruginosa. Med J Islamic World Acad Sci. 2006; 16(4): 171-179
Corresponding Author: M. D. Samsuzzaman, Bangladesh |
|
Copyright Transfer Form
Full Text PDF
Print
Download citation
RIS
Share with email
Share
Send email to author
PubMed