Antimicrobial Activity of Small Protein of Moringa Oleifera Leaves

Three fractions from the leaves of Moringa oleifera were obtained on Sephadex G-25 column chromatography. Single bands of these fractions were detected on Polyacrylamide SDS gel electrophoresis. An antibacterial action of small protein/peptide was tested against E. coli Kl. aerogenes, KI. pneumoniae, S. aureus, and B. subtilis. Fraction P1, P2 and P3 showed strong inhibitory activity against E. coli, S. aureus and B subtilis but clear zone of inhibition was also noted against K1. aerogenes with peptide 1. Fraction P2 showed ignificant zone of inhibition against Aspergillus niger.