The Effect of Verapamil and Magnesium Sulfate on Regional Cerebral Blood Flow in A Pup Model of Perinatal Sphyxia

The purpose of this study was to observe the effects of three minutes asphyxia on regional cerebral blood flow (rCBF) in newborn puppies. Another aim was to see the influence of verapamil and magnesium sulfate on rCBF. Blood pH, pO2, pCO2 were measured before and after asphyxia. Meanwhile, rCBF was measured by using single photon emission computerized tomography (SPECT) before and after asphyxia with 1 mCi Tc-99 m HMPAO and 2.5 mCi Tc-99 m HMPAO respectively. Then, the puppies were divided into three groups (verapamil, magnesium sulfate and serum physiologic). After administering the drugs intravenously, rCBFs of the puppies were measured by SPECT. The differences between basal and post-asphyxial pO2, pCO2 and pH values were significant. However, basal post-asphyxial and postdrug measurements for rCBF revealed no statistically significant differences. We therefore conclude that, three minutes asphyxia in puppies seem to be a safe period. Additionally verapamil and magnesium sulfate have not altered rCBF.