ISSN 2415-1297 (Online)   ISSN 2415-1300 (Print)
 
             
 
Volume : 27 Issue : 3 Year : 2019
 
Partial Purification of Phospholipase C from Oxystelma Esculantum [Med J Islamic World Acad Sci]
Med J Islamic World Acad Sci. 1993; 6(2): 95-98

Partial Purification of Phospholipase C from Oxystelma Esculantum

A. N. Memon, M. U. Dahot
From Department of Biochemistry, Institute of Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan.

Phospholipase C from Oxystelma esculantum was purified by acetone precipitation and
column chromatography on Sephadex G-100. It was then separated into two fractions (Phospholipase C I and II). Phospholipase C was homogeneous on polyacrylamide disc gel electrophoresis. The optimum pH of Phospholipase C I was 5.5 and the temperature 30C. The nature of phospholipase C I was that of a metalloenzyme activated in presence of CoCl2, ZnCl2 and CaCl2 whereas it was completely inhibited by EDTA due to chelation with metal ion. It was heatlabile.

Keywords: Oxystelma esculantum, tuber, phospholipase C.


A. N. Memon, M. U. Dahot. Partial Purification of Phospholipase C from Oxystelma Esculantum. Med J Islamic World Acad Sci. 1993; 6(2): 95-98

Corresponding Author: A. N. Memon, Pakistan


TOOLS
Full Text PDF
Print
Download citation
RIS
EndNote
BibTex
Medlars
Procite
Reference Manager
Share with email
Share
Send email to author

Similar articles
PubMed
Google Scholar




   
Copyright 2021 medicaljournal-ias.org. All Rights Reserved.