ISSN 2415-1297 (Online)   ISSN 2415-1300 (Print)
 
             
 
Volume : 27 Issue : 3 Year : 2019
 
Acridinium Ester Labelling Nd Characterization of Bovine Cardiac Troponin (cTn-I) Specific Monoclonal Antibodies [Med J Islamic World Acad Sci]
Med J Islamic World Acad Sci. 1996; 9(1): 3-8

Acridinium Ester Labelling Nd Characterization of Bovine Cardiac Troponin (cTn-I) Specific Monoclonal Antibodies

KH. H. Haider1, W. H. Stimson2
1From Faculty of Pharmacy, University of the Punjab (Old Campus), The Mall, Lahore-Pakistan.
2From Department of Immunology, University of Strathclyde, Glasgow- G4 ONR, Scotland (U.K).

The use of monoclonal antibody (McAb) based immunoassays to detect serum concentration changes of cardiac specific structural and regulatory proteins, as biochemical markers, is gaining immense interest in diagnostic procedures for cardiomyocyte necrosis. Of the non-isotopic probes, chemiluminescent molecules such as acridinium esters (A.E.) are regarded as being of real value in routine clinical chemistry. In the present study, bovine cardiac troponin-I specific 5F4 McAb was purified by Protein 'A' and Mono-Q ionexchange chromatography and conjugated with A.E. The method of purification hardly interfered with the conjugation procedure. The A.E. conjugated 5F4 retained its immunoreactivity post conjugation and was used to design direct and indirect immunoassays for the detection of cTn-I in vitro samples.

Keywords: Acridinium ester, Cardiac troponin-I, Monoclonal antibody.


KH. H. Haider, W. H. Stimson. Acridinium Ester Labelling Nd Characterization of Bovine Cardiac Troponin (cTn-I) Specific Monoclonal Antibodies. Med J Islamic World Acad Sci. 1996; 9(1): 3-8

Corresponding Author: KH. H. Haider, Pakistan


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