Detection and Characterization of A Heat Stable Bacteriocin (Lactocin Lc-09) Produced by A Clinical Isolate of LactobacilliFarida Khalid, Roquya Siddiqi, Naheed MojganiDepartment of Microbiology, University of Karachi, Karachi- 75270, Pakistan.
A new bacteriocin lactocin LC-09 produced by Lactobacillus strain LC-09, isolated from a clinical sample, was inhibitory against many species of lactobacilli and other Gram-positive bacteria including Listeria ivanovii, Streptococcus agalactii and S. peyogenes. Characterization of the bacteriocin revealed it to be extremely heat stable (4 hours at 100°C) and active at acidic pH values. This bacteriocin was inactivated by protease treatment. Curing of LC-09 with acridine orange, ethidium bromide and by elevated temperature (40°C) resulted in mutants defective in bacteriocin production. Keywords: Heat stable, inhibitory, protease.
Farida Khalid, Roquya Siddiqi, Naheed Mojgani. Detection and Characterization of A Heat Stable Bacteriocin (Lactocin Lc-09) Produced by A Clinical Isolate of Lactobacilli. Med J Islamic World Acad Sci. 1999; 12(3): 67-71
Corresponding Author: Roquya Siddiqi, Pakistan |
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