Extracellular Amylase Synthesis by Aspergillus Flavus and Penicillium Purpurescence

The potentiality of the two mold fungi A. flavus and P. purpurescence to produce extracellular amylase when grown on starch-containing medium as a sole carbon source in shaked flasks was tested. The maximal amylase productivity was achieved after 7 days incubation at 45°C. An initial pH value of 7 was found to be the optimum for amylolytic activity. Best activity was achieved upon using 2% (v/v) inoculum with 50 ml medium in 250 ml culture flask The greatest yield of amylolytic activity was obtained when starch or glycogen were used as carbon source. A starch concentration of 15% (w/v) favoured the highest enzyme activity, Ca+2 and Na+ ions showed stimulatory effect on enzyme productivity.