ISSN 2415-1297 (Online)   ISSN 2415-1300 (Print)
Volume : 30 Issue : 1 Year : 2023
Preparation and Application of Lectin-Gold Complexes [Med J Islamic World Acad Sci]
Med J Islamic World Acad Sci. 1994; 7(2): 120-129

Preparation and Application of Lectin-Gold Complexes

Aysel Şeftalioğlu, Hakkı Dalçık
From Department of Histology and Embryology, Medical School, Hacettepe University, Ankara, Türkiye.

The results obtained with various plant and animal tissues as well as pathogenic cells demonstrate that lectin-gold complexes enable localization of sugar residues at the ultra structural level. Provided that sugar residues are not altered during processing, the lectin- gold method represents undoubtedly a simple and powerful approach for localizing glyco-conjugates. Because of the electron dense gold marker, the labeling obtained is always of high resolution and a good correlation of markers and the cellular compartments can be easily and repeatedly established. In our studies, incubation of thin sections of the human conjunctival goblet cells having N. acetyl D-Galactoseamine rich mucus droplets with the HPA-GC revealed numerous gold particles in the electron micrographs.

Keywords: Lectin-gold complexes, gluco-conjugates, post-embedding.

Aysel Şeftalioğlu, Hakkı Dalçık. Preparation and Application of Lectin-Gold Complexes. Med J Islamic World Acad Sci. 1994; 7(2): 120-129

Corresponding Author: Aysel Şeftalioğlu, Türkiye

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