Cellulase Production from Actinomycetes Isolated from Iraqi Soils: II. Cell Growth and Cellulase Activity of Streptomyces Sp. Strain AT7 At Different TemperaturesAmira M. Al-Tai, Shatha Abdul-Razzak, Siham S. Al-Attiyah, Basima A. Abdul-NourFrom the Genetic Engineering and Biotechnology Research Centre, Scientific Research Council, P.O. Box 2250, Jadriyah, Baghdad, Iraq.
Streptomyces sp. Strain AT7 releases both carboxymethylcellulose (Cx) and Avicelase (C1) enzymes during growth in liquid medium with maximal activities, measured as mg/ml/h, of 6.0 and 13.6 obtained at 36ºC for 2 and 9 day incubation periods respectively. Cx kept its activity at 42ºC till the end of the incubation period (18 days) while C1 lost almost 50% of its activity at the same time. Maximum yields of cell growth (protein) obtained at 28º, 36º and 42ºC; were 0.23, 0.22 and 0.25 g/ml respectively. Keywords: Streptomycetes, Cellulase Activity, Carboxymethylcellulase, Avicelase.
Amira M. Al-Tai, Shatha Abdul-Razzak, Siham S. Al-Attiyah, Basima A. Abdul-Nour. Cellulase Production from Actinomycetes Isolated from Iraqi Soils: II. Cell Growth and Cellulase Activity of Streptomyces Sp. Strain AT7 At Different Temperatures. Med J Islamic World Acad Sci. 1989; 2(3): 185-188
Corresponding Author: Amira M. Al-Tai, Iraq |
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